Does this antibody recognize native MUC5AC?

Yes, this antibody has been tested positive against native human MUC5AC antigen (M1 mucin preparation). It is a Recombinant monoclonal antibody (Chimeric mouse/human) that binds specifically to the 5AC antigen found in gastric and colorectal tissues.

What applications is this antibody suitable for?

This antibody is suitable for a wide range of applications, including Immunoprecipitation (IP), Immunofluorescence (IF), Functional Studies (FuncS), Flow Cytometry (FC), Neutralization (Neut), ELISA, and Immunocytochemistry (ICC).

Anti-Human MUC5AC Recombinant Antibody (TAB-200)

CAT#: TAB-200

Recombinant monoclonal antibody to 5AC. This antibody is a chimeric monoclonal antibody is suitable for use in IP, IF, FuncS, FC, Neut, ELISA, ICC and most other immunological methods.

Tested Data Gene Expression

SDS-PAGE

Figure 1 Anti-Human MUC5AC Recombinant Antibody (TAB-200) in SDS-PAGE

Lane 1: Non-reduced mAb
Lane 2: Reduced mAb

SEC-HPLC

Figure 2 Anti-Human MUC5AC Recombinant Antibody (TAB-200) in SEC-HPLC

The purity of TAB-200 was greater than 95% as determined by SEC-HPLC.

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Normal Tissue Figure 1 High expression in stomach 1

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40615/124256_A_6_1_val_selected.jpg

Normal Tissue Figure 2 Low expression in liver

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40615/124256_A_9_4_val_selected.jpg

Normal Tissue Figure 3 Stomach

Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40615/124256_A_3_1.jpg

RNA Expression Figure 4 RNA cell line category: Group enriched (A549, OE19)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000215182-MUC5AC

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Specifications

Immunogen
M1 mucin preparation from the fluid of an ovarian mucinous cyst.

Host Species
Mouse

Derivation
Chimeric (mouse/human)

Type
IgG1 - kappa

Specificity
Tested positive against native human antigen.

Species Reactivity
Human

Applications
Suitable for use in IP, IF, FuncS, FC, Neut, ELISA, ICC and most other immunological methods.

MW
142.8 kDa

Related Disease
Colorectal cancers

Product Property

Purity
>95.0% as determined by analysis by RP-HPLC.

Storage
At -20°C for one year.

Target

Alternative Names
MUC5AC; mucin 5AC, oligomeric mucus/gel-forming; mucin 5, subtypes A and C, tracheobronchial/gastric; mucin-5AC; MUC5; gastric mucin; tracheobronchial mucin; major airway glycoprotein; lewis B blood group antigen; mucin-5 su

Gene ID
4586

UniProt ID
P98088

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Breast cancer biomarkers at key points during disease progression

Excellent MUC5AC Detection

We study mucins in colorectal cancer, and this chimeric (mouse/human) antibody is highly specific for MUC5AC. We used it for Immunoprecipitation and Immunofluorescence, and it effectively detected the antigen in our samples. It is a robust tool for investigating mucin glycosylation changes.

Versatile Functional Antibody

The purity of this antibody (>95% by RP-HPLC) is impressive. We successfully used it in functional studies (FuncS) and Neutralization assays. Its ability to work in multiple applications, including ICC and FC, makes it a versatile reagent for our comprehensive mucin characterization projects.

Q&As

Does this antibody recognize native MUC5AC?

A: Yes, this antibody has been tested positive against native human MUC5AC antigen (M1 mucin preparation). It is a Recombinant monoclonal antibody (Chimeric mouse/human) that binds specifically to the 5AC antigen found in gastric and colorectal tissues.
What applications is this antibody suitable for?

A: This antibody is suitable for a wide range of applications, including Immunoprecipitation (IP), Immunofluorescence (IF), Functional Studies (FuncS), Flow Cytometry (FC), Neutralization (Neut), ELISA, and Immunocytochemistry (ICC).

View the frequently asked questions answered by Creative Biolabs Support.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-200, RRID: AB_3111869)

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Biosimilar Overview

Please refer to Ensituximab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Ensituximab.

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-200.pdf

MSDS

MSDS - TAB-200.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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